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Microscopy

The bio-imaging technical platform provides CR-HMR researchers with cutting-edge optical microscopy equipment to acquire images or videos of living or fixed samples. It also provides different types of software for microscopic image analysis. It offers training sessions as well as support in the design of microscopy experiments.

Equipment

Olympus FV1000 LSM inverted confocal/multiphoton system

  • Olympus IX81 motorized inverted microscope
  • Olympus FluoView FV1000 scan unit with spectral detectors
  • Laser fusion module with AOTF lasers on order: 405 nm, 458 nm, 488 nm, 515 nm, 543 nm, and 635 nm
  • SpectraPhysics Mai Tai DeepSee Ti: Pulsed femtosecond sapphire laser
  • 2 NDD PMT detectors
  • QImaging Retiga EX monochrome CCD digital camera
  • ProScan III linear motor positioning stage
  • HBO 100 lamp
  • Vibration isolation optical table
  • Olympus FluoView and cellSens software

Zeiss LS M880 upright multiphoton system

  • Zeiss Axio Examiner.Z1 upright motorized microscope
  • Zeiss LS M880 scan unit with spectral detectors
  • SpectraPhysics Mai Tai DeepSee Ti: Pulsed femtosecond sapphire laser
  • Ultra-deep imaging with water-immersion objective W Plan-Apo 20x/1.0 WD = 1.8
  • High-speed attenuation via AOM 
  • 4 multi-alkali NDD PMT detectors
  • Prior Proscan linear motor positioning stage
  • HXP120 metal halide illumination source
  • Warner Instruments perfusion microincubator
  • Vibration isolation optical table
  • Zeiss Zen Black and Zen Blue Software

CPN Zeiss System laser microdissection system

  • Zeiss PALM with a 350-nm solid state laser
  • Zeiss Axio Observer.Z1 motorized inverted microscope
  • Zeiss Apotome for optical sectioning
  • X-Cite metal halide illumination source
  • Axiocam MRm CCD monochrome camera and AxioCam ICc1 colour camera
  • Linear motor positioning stage
  • Zeiss AxioVision and Palm RoboSoftware

DeltaVision Elite Imaging System

  • Olympus IX71 inverted microscope with high-speed motorized filter wheels
  • UltimateFocus focus correction system
  • High-precision linear motor positioning stage
  • Incubation chamber for long-term live-cell imaging
  • LED lamp
  • Monochrome sCMOS camera
  • Deconvolution for optical sectioning
  • GE SoftWoRx software

Zeiss live-cell imaging system

  • Zeiss Axio Observer.Z1 motorized inverted microscope
  • PeCon S incubation chamber for long-term live-cell imaging
  • TempModule S and CO2 Module S
  • High-precision linear motor positioning stage
  • AxioCam MRm CCD monochrome camera
  • LED lamp
  • Zen Blue image acquisition software
     

Zeiss motorized wide-field imaging system

  • Zeiss motorized imaging system
  • Zeiss Axio Imager.Z2 upright motorized microscope
  • AxioCam MRm CCD monochrome camera and AxioCam MRc colour camera 
  • High-precision linear motor positioning stage
  • LED lamp
  • Zeiss AxioVision 3D deconvolution software

Zeiss ratiometric wide-field imaging system

  • Zeiss Axio Observer.A1 inverted microscope
  • Sutter high-speed DG-5 xenon arc lamp for ratiometric imaging 
  • Sutter Lambda 10-3 high-speed filter wheel changers
  • Tokai Hit incubation chamber for long-term live-cell imaging
  • QImaging Retiga EX monochrome CCD digital camera
  • MetaMorph image acquisition software

Zeiss inverted wide-field imaging system

  • Zeiss Axio Observer.A1 inverted microscope
  • Sutter high-speed DG-5 xenon arc lamp for ratiometric imaging
  • Sutter Lambda 10-3 high-speed filter wheel changers
  • Photometrics CoolSnap CCD monochrome camera
  • MetaMorph image acquisition software

Olympus inverted wide-field imaging system

  • Olympus IX73 inverted microscope
  • Olympus U-HGLGPS 130W light source
  • Olympus SC30 colour CCD camera and XM10 monochrome camera
  • Olympus cellSens software

Système d’imagerie Olympus widefield inversé — niveau de confinement 2

  • Olympus IX73 microscope inversé
  • Olympus U-HGLGPS 130W source lumineuse
  • Caméras CCD Olympus SC30 couleur et XM10 monochrome
  • Logiciels Olympus cellSens

Olympus inverted wide-field imaging system – Containment Level 2

  • Olympus IX73 inverted microscope
  • Olympus U-HGLGPS 130W light source
  • Olympus SC30 colour CCD camera and XM10 monochrome camera
  • Olympus cellSens software

Olympus upright wide-field imaging system

  • Olympus IX73 inverted microscope
  • Olympus U-HGLGPS 130W light source
  • Olympus XM10 CCD monochrome camera and SC30 colour camera
  • Olympus cellSens software

Olympus upright wide-field imaging system

  • Leica DMRE upright microscope
  • HBO 100 lamp
  • QImaging QCam CCD monochrome camera
  • Micro-Manager software

Nikon upright wide-field imaging system

  • Nikon Eclipse E800 upright microscope
  • Photometrics CoolSnap cf CCD monochrome camera
  • Prior Proscan high-precision linear motor positioning stage 
  • HBO 100 lamp
  • Micro-Manager software

Coordinator

Mikhail Sergeev, PhD
514-252-3400